https://biobran.org Sun, 23 Aug 2026 14:45:43 +0000 en-US hourly 1 https://wordpress.org/?v=7.1 https://biobran.org/wp-content/uploads/2026/08/cropped-FaviBB-1-32x32.webp https://biobran.org 32 32 https://biobran.org/synergistic-role-of-arabinoxylan-rice-bran-mgn-3-biobran-in-s-cerevisiae-induced-apoptosis-of-monolayer-breast-cancer-mcf-7-cells-peer-reviewed/ Sun, 14 Aug 2005 17:56:00 +0000 https://biobran.org/?p=585 We have recently demonstrated that breast cancer cell (BCC) lines undergo apoptosis following phagocytosis of S. cerevisiae. Arabinoxylan rice bran extract (MGN-3/Biobran) has been shown to enhance this effect. Since previous data were obtained using cells in suspension, the present study was undertaken to examine monolayer BCC that more closely model cancer cell growth. Monolayers of both breast cancer (MCF-7) and non-tumorgenic breast epithelial (MCF-10A) cells grown on glass coverslips were cultured with heat-killed S. cerevisiae at a ratio of 1:10, respectively. MCF-7 cells phagocytized yeast in a time-dependent manner, 6.9% to 14.3% from 1 h to 4 h, respectively, with a 2-fold increase in the presence of MGN-3. On the other hand, there was virtually no phagocytosis of yeast by MCF-10A cells. Similarly, yeast-induced apoptosis of MCF-7 cells occurred in a time-dependent manner from 11.5% after 1 h to 21. 7% after 4 h, and was enhanced in the presence of MGN-3. These data may have implications in the treatment of breast cancer.

]]> https://biobran.org/modified-arabinoxylan-rice-bran-mgn-3-biobran-enhances-yeast-induced-apoptosis-in-human-breast-cancer-cells-in-vitro/ Sun, 14 Aug 2005 17:52:00 +0000 https://biobran.org/?p=582 We have recently reported that phagocytosis of killed Saccharomyces cerevisiae, baker’s yeast, induced apoptosis in human breast cancer cell lines MCF-Z, ZR- 75-1 and HCC70. In this study we have evaluated the ejfect of treatment with MGN-3, a modified arabinoxylan from rice bran, on phagocytosis and yeast-induced apoptosis in breast cancer cells. Cancer cells were cultured with yeast at a ratio of 1:10 in the absence or presence of MGN-3, and the percentages of phagocytic and apoptotic cancer cells were examined by flow cytometry and by cytospin preparations. Cancer cells treated with MGN-3 exhibited increased percentages of attachment (200%) and uptake of yeast (313%) by MCF-7 cells at 0.5 hr, as compared with cells without MGN-3. In addition, treatment with MGN-3 resulted in a 2 fold increase in the percentage of apoptotic MCF-7 cells, 2.5 fold for ZR-75 cells and 1.8 fold for HCC70 cells. MGN-3 effect was dose-dependent and associated with increased activation of caspases 8 and 9 in MCF-7 cells, and caspases 8, 9 and 3 in HCC70 cells. This data demonstrates that MGN-3 accelerates phagocytosis-induced apoptosis of cancer cells, which may represent a novel therapeutic strategy for the treatment of breast cancer.

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