peer – Biobran

Basophilic Response in Patients with Persistent Symptoms Attributed to Lyme Borreliosis Treated with Hydrolysed Arabinoxylan Rice Bran

admin — Sat, 16 Aug 2025 20:11:00 +0000

Background and Objectives: MGN-3/Biobran (BRM4, Lentin Plus or Ribraxx) is a natural, rice bran-derived arabinoxylan immunoceutical that modulates the adaptive immune response to viral infections. In response to bacterial infections, basophils act as “first responders” and are also associated with modulation of the adaptive immune response. The maturation of pluripotent CD34+ stem cells into basophils is supported by the cytokine interleukin-3 (IL-3). The aim was to test the hypothesis that modulation of the adaptive immune response in bacterial infection by MGN-3/Biobran entails a basophilic response. The tick-related disorder Lyme borreliosis was chosen as the disease model; tick bites are associated with cutaneous IL-3-mediated basophil recruitment.

Materials and Methods: A three-month randomised double-blind placebo-controlled trial was conducted in patients with a history of borreliosis who were suffering from symptoms attributable to this disorder. The immunoceutical group received oral Biobran; the dosage for both groups was 1 g thrice daily. Both groups were matched for age, sex, and ethnicity. Results: A higher percentage of basophil count occurred in the immunoceutical group (p = 0.038). The final general linear model included the group (immunoceutical/placebo) and change in fatigue assessed by the 11-item Chalder Fatigue Questionnaire (CFQ) (r2 = 0.63; p = 0.0066). The change in basophil count was positively correlated with CFQ change (rs = 0.633; p = 0.020); only the immunoceutical group showed a positive correlation.

Conclusions: These results support the hypothesis being tested. Basophils may modulate the adaptive immune response by acting as immunoregulatory cells. They can regulate the functioning of type 2 T-helper lymphocytes, enhance immunological memory, and present antigens to CD8 T lymphocytes. Further studies are needed to clarify potential mechanistic factors and the timing of this basophilic response

Dieatary Supplementation with BioBran-MGN-3 Increases Innate Resistance and Reduces the Incidence of Influenza-like Illnesses s in Elderly Subjects: A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Trial

admin — Mon, 16 Aug 2021 19:45:00 +0000

Abstract: Influenza-like illness (ILI) remains a major cause of severe mortality and morbidity in the elderly. Aging is associated with a decreased ability to sense pathogens and mount effective innate and adaptive immune responses, thus mandating the development of protective nutraceuticals. Biobran/MGN-3, an arabinoxylan from rice bran, has potent anti-aging and immunomodulatory effects, suggesting that it may be effective against ILI. The objective of the current study was to investigate the effect of Biobran/MGN-3 on ILI incidence, natural killer (NK) cell activity, and the expressions of RIG-1 (retinoic acid-inducible gene 1), MDA5 (melanoma differentiation-associated protein 5), and their downstream signaling genes ISG-15 (interferon-stimulated genes 15) and MX1 (myxovirus (influenza) resistance 1, interferon-inducible). A double-blind, placebo-controlled clinical trial included eighty healthy older adults over 55 years old, 40 males and 40 females, who received either a placebo or Biobran/MGN-3 (500 mg/day) for 3 months during known ILI seasonality (peak incidence) in Egypt. The incidence of ILI was confirmed clinically according to the WHO case definition criteria. Hematological, hepatic, and renal parameters were assessed in all subjects, while the activity of NK and NKT (natural killer T) cells was assessed in six randomly chosen subjects in each group by the degranulation assay. The effect of Biobran/MGN-3 on RIG-1 and MDA5, as well as downstream ISG15 and MX1, was assessed in BEAS-2B pulmonary epithelial cells using flow cytometry. The incidence rate and incidence density of ILI in the Biobran/MGN-3 group were 5.0% and 0.57 cases per 1000 person-days, respectively, compared to 22.5% and 2.95 cases per 1000 person-days in the placebo group. Furthermore, Biobran/MGN-3 ingestion significantly enhanced NK activity compared to the basal levels and to the placebo group. In addition, Biobran/MGN-3 significantly upregulated the expression levels of RIG-1, MDA5, ISG15, and MX1 in the human pulmonary epithelial BEAS-2B cell lines. No side effects were observed. Taken together, Biobran/MGN-3 supplementation enhanced the innate immune response of elderly subjects by upregulating the NK activity associated with reduction of ILI incidence. It also upregulated the intracellular RIG-1, MDA5, ISG15, and MX1 expression in pulmonary epithelial tissue cultures. Biobran/MGN-3 could be a novel agent with prophylactic effects against a wide spectrum of respiratory viral infections that warrants further investigation.

Rice bran arabinoxylan compound and quality of life of cancer patients (RBAC – QoL): Study protocol for a randomized pilot feasibility trial

admin — Sun, 16 Aug 2020 19:57:00 +0000

Introduction : Rice bran arabinoxylan com pound (RBAC) is a nutraceutical for enhancing a depleted immune system during and after cancer treatment. This pilot feasibility trial aims to evaluate the effects of RBAC on cancer patients’ quality of life during active treatment, compared to placebo, using a validated questionnaire. Other outcome measures include changes in inflammatory and nutritional status, cytokine pro file, and gut mi – crobiota. Methods/ Design : The study will recruit 50 participants from a regional cancer center in Australia. Patients aged 18 –70, diagnosed with solid or an cancers stage II and above, and currently undergoing active systemic thera – pies, are eligible. Random allocation of participants into two groups is stratified based on metastatic status and treatment type. The dosage is either 3 g/ day of RBAC or placebo in identical packaging. The participants, study coordinator, and treating oncologists are blinded to the interventions. Data collections are at base line and at four follow – up sessions, which are six weeks apart (24 weeks). Statistical analysis will in volve a pro – tected p – value with multiple dependent values and analyzed by ANOVA with repeated measures on the occa – sion of testing and with both a full Bonferroni or Sidak corrections applied to protect against Type I errors. Any observed significance war rants further analysis with pair wise comparisons. Analysis of covariance will also be performed to assess any in fluence of the demographic data, cancer diagnosis, as well as changes in physical activity, dietary habits, and complementary medicine usage. Comparisons of gut microbiota will be based on the analysis of the fecal microbiome using 16S ribosomal ribonucleic acid amplicon sequencing. The proposed research time line is from October 2018 to May 2022. Trial registration : ANZCTR. Reg No: AC TRN12619000562178p.

The Immunomodulating Effects of Arabinoxylan Rice Bran (Lentin) on Hematologic Profile, Nutritional Status and Quality of Life among Head and Neck Carcinoma Patients Undergoing Radiation Therapy: A Double Blind Randomized Control Trial

admin — Sun, 16 Aug 2020 19:38:00 +0000

Immunostimulants have been explored to reduce the complications of radiation/chemotherapy. This double blind randomized trial aimed to determine the immunomodulating effects of Lentin among head and neck cancer patients in addressing radiation treatment complications such as anemia, leukopenia, weight loss and improvement of quality of life. Sixty-five (65) patients were enrolled and given either Lentin or placebo – 2 weeks prior, during radiation/chemoradiotherapy and 2 months after. Complete Blood Count, Body Mass Index, percent weight loss and EORTC Quality of Life questionnaires QLQ H&N35 were used to assess the degree of anemia, weight loss and quality of life. Overall CBC results revealed higher values on all parameters in Lentin arm. Upon completion of radiochemotherapy, the Lentin arm showed significantly higher mean hemoglobin by 1.30 g/dL (p=0.010), hematocrit (p=0.001), RBC (p=0.001) and platelets (p=0.017). Also, higher overall BMI (22.69 versus 21.52) and a lower percent weight loss (6.10% versus 6.91%) for Lentin compared to placebo were noted with p-values of 0.199 and 0.571, respectively. Treatment related toxicity using the RTOG grading showed lower severity scores on all parameters (p-values: >0.05) and better QoL scores for patients taking Lentin (p-value: 0.019). Results from this study showed better clinical outcomes for patients taking Lentin. These have led to fewer blood transfusions, treatment delays and hospital admissions, avoidance of treatment mortalities and morbidities, and improved quality of life among head and neck cancer patients undergoing chemoradiotherapy.

Arabinoxylan rice bran (MGN-3/Biobran) alleviates radiation-induced intestinal barrier dysfunction of mice in a mitochondrion-dependent manner

admin — Sun, 16 Aug 2020 19:35:00 +0000

MGN-3 is an arabinoxylan from rice bran that has been shown to be an excellent antioxidant and radioprotector. This study examined the protective effects of MGN-3 on radiation-induced intestinal injury. Mice were treated with MGN-3 prior to irradiation, then continued to receive MGN-3 for 4 weeks thereafter. MGN-3 increased the activity of mitochondrial respiratory chain complexes Ⅰ, Ⅲ, Ⅳ and Ⅴ, the intercellular ATP content, the mitochondria-encoded gene expression and mitochondrial copy numbers in the jejunal and colonic mucosa. MGN-3 reduced the oxidative stress levels and inflammatory response indicators in the serum and jejunal and colonic mucosa. Antioxidant indicators such as superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity were significantly increased in the serum and jejunal and colonic mucosa in the MGN-3 group. Moreover, MGN-3 decreased the gene abundances and enzymatic activities of caspase-3, 8, 9 and 10 in the jejunal and colonic mucosa. The endotoxin, diamine peroxidase, D-lactate and zonulin levels were significantly reduced in the serum and jejunal and colonic mucosa in the MGN-3 group. MGN-3 also markedly upregulated the gene abundances of ZO-1, occludin, claudin-1 and mucin 2. MGN-3 effectively attenuated radiation-induced changes in the intestinal epithelial mitochondrial function, oxidative stress, inflammatory response, apoptosis, intestinal permeability and barrier function in mice. These findings add to our understanding of the potential mechanisms by which MGN-3 alleviates radioactive intestinal injury.

Chemopreventive role of arabinoxylan rice bran, MGN-3/Biobran, on liver carcinogenesis in rats

admin — Sun, 16 Aug 2020 19:33:00 +0000

Hepatocellular carcinoma (HCC) is one of the most common cancers in the world and one of the most lethal. MGN-3/Biobran is a natural product derived from rice bran hemicelluloses and has been reported to possess a potent anticancer effect in a clinical study of patients with HCC. The current study examines the mechanisms by which Biobran protects against chemically induced hepatocarcinogenesis in rats. The chemical carcinogen used in this study is N-nitrosodiethylamine (NDEA) plus carbon tetrachloride (CCl4). Rats were treated with this carcinogen, and the animals were pretreated or posttreated with Biobran via intraperitoneal injections until the end of the experiment. Treatment with Biobran resulted in: 1) significant alleviation of liver preneoplastic lesions towards normal hepatocellular architecture in association with inhibition of collagen fiber deposition; 2) arrest of cancer cells in the sub-G1 phase of the cell cycle; 3) increased DNA fragmentation in cancer cells; 4) down-regulated expression of Bcl-2 and up-regulated expression of p53, Bax, and caspase-3; and 5) protection against carcinogen-induced suppression of IkappaB-alpha (IκB-α) mRNA expression and inhibition of nuclear factor kappa-B (NF-κB/p65) expression. Additionally, the effect of Biobran treatment was found to be more significant when supplemented prior to carcinogen-induced hepatocarcinogenesis as compared to posttreatment. We conclude that Biobran inhibits hepatocarcinogenesis in rats by mechanisms that include induction of apoptosis, inhibition of inflammation, and suppression of cancer cell proliferation. Biobran may be a promising chemopreventive and chemotherapeutic agent for liver carcinogenesis

Controlled pilot study for cancer patients suffering from chronic fatigue syndrome due to chemotherapy treated with BioBran (MGN-3- Arabinoxylane) and targeted radiofrequency heat therapy

admin — Tue, 16 Aug 2016 19:09:00 +0000

Introduction: Although modern therapies for cancer have improved life expectancy, the management of disease and improvement of quality of life (QoL) of patients, especially managing cancer-related pain and chronic fatigue syndrome are still limited. We demonstrate the efficacy of a combined therapy to treat cancer patients suffering from CFS. The effects of a combined therapy in cancer patients suffering from CFS was evaluated. NK cells were stimulated, additional tumour treatment together with targeted radiofrequency therapy (Oncothermia).

Methods: SIXTY patients with CFS (due to suffering from any type of cancer) were recruited, (according to the Centres for Disease Control 1994 criteria) attending an outpatient specialist CFS service for controlled pilot study. A total of 25 participants were given oral BioBran (MGN-3-Arabinoxylane), + Oncothermia, for six months, equivalent control group has not received this complex (BioBran + Oncothermia) treatment, they received chemo-,radiotherapy treatment.

Results: The whole body pH status showed strong tissue acidity before the treatment, but the BioBran group changed the tissue pH status. The most important finding was that the average of CFQ score was significantly reduced after the treatment, and in control group the CFQ scores did not change significantly.

Conclusion: The findings support a specific therapeutic effect of the complex BioBran+ Oncothermia therapy in CFS of cancer patients improving their QoL, enhancing NK activity in synergy.

Home Products Research Distributors Contact Can a synergistic activation of pattern recognition receptors by plant immunomodulators enhance the effect of oncologic therapy? Case Report of a patient with uterus and ovary sarcoma

admin — Sun, 16 Aug 2015 18:11:00 +0000

Background: Growing evidence supports the hypothesis that similar to microbes various plant extracts can also contain Pathogenic Associated Molecular Pattern (PAMP)-like structures which can activate type-1 cellular functions of the innate immune system. Since they are important in tumor defense and the chemical production of PAMP structures is hardly accomplishable, the plant extracts standardized concerning their PAMP like structures may be promising for future tumor therapy. Method: The synergistic effect of two standardized plant immunomodulators was monitored by the hemocytological measurement of the peripheral level of Natural Killer (NK) cells. Suboptimal doses of istletoe lectins (ML) and Arabinoxylan in MGN-3 were compared using healthy volunteers. Results: 24h after a single suboptimal dose (15 mg/kg) of Arabinoxylan in MGN-3 an average increase (+/-SEM) in NK level was 46.4 (+/-36)% and 24h after a single suboptimal (0.45 ng/kg) ML injection a 36 (+/-13) % enhancement was found. If these suboptimal doses of Arabinoxylan and ML were given together, a highly significant enhancement (293 +/-41%) was established indicating a high significant synergism between them (p<0.001). A patient with uterus and ovary sarcoma was not able to tolerate the CYVADIC chemotherapy. After its combination with ML and Arabinoxylan using optimal doses: 0.75 ng/kg and 45 mg/kg respectively, she received six cycles CYVADIC and thereafter only immunotherapy was given. During the following five years she has regularly been tumor free. Conclusion: The combination of standardized plant extracts with PAMP-like structures seems to open new perspectives in the supportive therapy of metastatic tumors. Further research is necessary.

Arabinoxylan rice bran (MGN-3/Biobran) enhances natural killer cell — mediated cytotoxicity against neuroblastoma in vitro and in vivo

admin — Sun, 16 Aug 2015 18:09:00 +0000

Background aims. Natural killer cell (NK) cytotoxic activity plays a major role in natural immunologic defences against malignancies. NK cells are emerging as a tool for adoptive cancer immunotherapies. Arabinoxylan rice bran (MGN-3/Biobran) has been described as a biological response modifier that can enhance the cytotoxic activity of NK cells. This study evaluated the effect of MGN-3/Biobran on NK cell activation, expansion and cytotoxicity against neuroblastoma cells. Methods. NK cells were enriched with magnetic beads and stimulated with MGN-3/Biobran. NK cell activation was evaluated via analysis of their phenotype, and their expansion capability was tracked. The in vitro cytotoxic ability of the activated NK cells was tested against K562, Jurkat, A673, NB1691, A-204, RD and RH-30 cell lines and the in vivo cytotoxic ability against the NB1691 cell line. Results. MGN-3/Biobran stimulation of NK cells induced a higher expression of the activationassociated receptors CD25 and CD69 than in unstimulated cells (P < 0.05). The expression of NKG2D, DNAM, NCRs and TLRs remained unchanged. Overnight MGN-3/Biobran stimulation increased NK cell cytotoxic activity against all cell lines tested in vitro and decelerated neuroblastoma growth in vivo. The mechanism is not mediated by lipopolysaccharide contamination in MGN-3/Biobran. Furthermore, the addition of MGN-3/Biobran promoted NK cell expansion and decreased T cells in vitro. Conclusions. Our data show that MGN-3/Biobran upregulates NK cell activation markers, stimulates NK cell cytotoxic activity against neuroblastoma in vitro and in vivo and selectively augments the expansion of NK cells. These results may be useful for future NK cell therapeutic strategies of the treatment of neuroblastoma.

MGN-3/BIOBRAN Enhances Generation of Cytotoxic CD8+ T Cells Via Upregulation of DEC-205 Expression on Dendritic Cells

admin — Sat, 16 Aug 2014 18:07:00 +0000

Arabinoxylan rice bran (MGN-3/Biobran) has been shown to be a potent biological response modifier (BRM) that activates different arms of the immune system, including dendritic cells (DCs), which prime CD4+ helper T-cell responses. The present study explores the ability of MGN-3-activated DCs to prime CD8+ T cells and examines the mechanisms underlying its effect. Human monocyte-derived DCs were treated with MGN-3 (20 and 40 µg/ml). Results indicate that treatment with MGN-3 caused DCs to prime higher granzyme B-expressing CD8+ T cells. Tumor lysate-pulsed MGN-3 DC also increased tumor cell killing compared to DC-stimulated CD8+ T cells. This was associated with: i) increased expression of DEC-205 in MGN-3-activated DCs in a dose—dependent manner; and ii) MGN-3 induced significant production of Type III interferon, IL29, but not Type I IFNs on and B. These results suggest that MGN-3 is a potent natural adjuvant that efficiently activates DCs and may therefore be useful for mounting an efficient immune response against infections and cancer.